Publication: Subpopulations of Staphylococcus aureus clonal complex 121 are associated with distinct clinical entities.
Accepted Date
2013-02-04
Issued Date
2013
Copyright Date
2013
Resource Type
Language
eng
ISSN
1932-6203 (electronic)
Rights
Mahidol University
Rights Holder(s)
Plos One
Bibliographic Citation
Kurt K, Rasigade JP, Laurent F, Goering RV, Žemličková H, Machova I, et al. Subpopulations of Staphylococcus aureus clonal complex 121 are associated with distinct clinical entities. PLoS One. 2013;8(3):e58155.
Suggested Citation
Kurt, Kevin, Rasigade, Jean-Philippe, Laurent, Frederic, Goering, Richard V, Zˇemlicˇkova, Helena, Machova, Ivana, Struelens, Marc J., Zautner, Andreas E., Holtfreter, Silva, Broker, Brarbaa, Ritchie, Stephen, Reaksmey, Sin, Direk Limmathurotsakul, ดิเรก ลิ้มมธุรสกุล, Peacock, Sharon J., Cuny, Christiane, Layer, Franziska, Witte, Wolfgang, Nubel, Ulrich Subpopulations of Staphylococcus aureus clonal complex 121 are associated with distinct clinical entities.. Kurt K, Rasigade JP, Laurent F, Goering RV, Žemličková H, Machova I, et al. Subpopulations of Staphylococcus aureus clonal complex 121 are associated with distinct clinical entities. PLoS One. 2013;8(3):e58155.. doi:10.1371/journal.pone.0058155 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/750
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Title
Subpopulations of Staphylococcus aureus clonal complex 121 are associated with distinct clinical entities.
Author(s)
Kurt, Kevin
Rasigade, Jean-Philippe
Laurent, Frederic
Goering, Richard V
Zˇemlicˇkova, Helena
Machova, Ivana
Struelens, Marc J.
Zautner, Andreas E.
Holtfreter, Silva
Broker, Brarbaa
Ritchie, Stephen
Reaksmey, Sin
Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Peacock, Sharon J.
Cuny, Christiane
Layer, Franziska
Witte, Wolfgang
Nubel, Ulrich
Rasigade, Jean-Philippe
Laurent, Frederic
Goering, Richard V
Zˇemlicˇkova, Helena
Machova, Ivana
Struelens, Marc J.
Zautner, Andreas E.
Holtfreter, Silva
Broker, Brarbaa
Ritchie, Stephen
Reaksmey, Sin
Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Peacock, Sharon J.
Cuny, Christiane
Layer, Franziska
Witte, Wolfgang
Nubel, Ulrich
Corresponding Author(s)
Other Contributor(s)
Abstract
We investigated the population structure of Staphylococcus aureus clonal complex
CC121 by mutation discovery at 115 genetic housekeeping loci from each of 154
isolates, sampled on five continents between 1953 and 2009. In addition, we
pyro-sequenced the genomes from ten representative isolates. The genome-wide SNPs
that were ascertained revealed the evolutionary history of CC121, indicating at
least six major clades (A to F) within the clonal complex and dating its most
recent common ancestor to the pre-antibiotic era. The toxin gene complement of
CC121 isolates was correlated with their SNP-based phylogeny. Moreover, we found
a highly significant association of clinical phenotypes with phylogenetic
affiliations, which is unusual for S. aureus. All isolates evidently sampled from
superficial infections (including staphylococcal scalded skin syndrome, bullous
impetigo, exfoliative dermatitis, conjunctivitis) clustered in clade F, which
included the European epidemic fusidic-acid resistant impetigo clone (EEFIC). In
comparison, isolates from deep-seated infections (abscess, furuncle, pyomyositis,
necrotizing pneumonia) were disseminated in several clades, but not in clade F.
Our results demonstrate that phylogenetic lineages with distinct clinical
properties exist within an S. aureus clonal complex, and that SNPs serve as
powerful discriminatory markers, able to identify these lineages. All CC121
genomes harboured a 41-kilobase prophage that was dissimilar to S. aureus phages
sequenced previously. Community-associated MRSA and MSSA from Cambodia were
extremely closely related, suggesting this MRSA arose in the region.