Publication: Triploid Penaeus monodon: Sex ratio and growth rate
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Issued Date
2012-08-01
Resource Type
ISSN
00448486
Other identifier(s)
2-s2.0-84863309632
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Mahidol University
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SCOPUS
Bibliographic Citation
Aquaculture. Vol.356-357, (2012), 7-13
Suggested Citation
Pattira Pongtippatee, Karemah Laburee, Pinij Thaweethamsewee, Ratana Hiranphan, Somluk Asuvapongpatana, Wattana Weerachatyanukul, Theera Srisawat, Boonsirm Withyachumnarnkul Triploid Penaeus monodon: Sex ratio and growth rate. Aquaculture. Vol.356-357, (2012), 7-13. doi:10.1016/j.aquaculture.2012.06.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13421
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Title
Triploid Penaeus monodon: Sex ratio and growth rate
Abstract
This study seeks to determine the best method for preventing the second body (PBII) extrusion as a means to indu ce triploidy in the black tiger shrimp Penaeus monodon. Chemical (cytochalasine-B and 6-dimethylaminopurine) and temperature (heat and cold) shocks were applied to newly fertilized eggs. Cold shock that was administered at 8 °C, for 10. min at 8. min post-spawning, was the best method for PBII triploidy induction, as evidenced by the highest percentage of forming three pronuclei in syngamy. Therefore, cold shock induction was employed to further explore the advantage of triploid over diploid shrimp. After hatching of the cold-shocked eggs, the larvae were allowed to reach juvenile and adult levels of development. The number and amount of chromosomes were determined in juveniles and adult stages, using Fluorescence Activating Cell Sorting methods, by which the shrimp were divided into diploid and triploid groups. At day 150 in culture, the average body weight of the triploid females (35.2 ± 3.2. g) and triploid males (31.5 ± 3.5. g) was significantly higher (P < 0.0001) than that of their diploid counterparts (24.5 ± 0.5. g for females and 23.1 ± 3.8. g for males), having a ratio of 2 females:1 male for triploid shrimp, and 2 females:3 males for diploid shrimp. These results reveal the advantages of growing triploid over diploid P. monodon, and its feasibility for commercial production. © 2012 Elsevier B.V.