Publication: Antimicrobial disk susceptibility testing of leptospira spp. Using leptospira vanaporn wuthiekanun (LVW) agar
1
Issued Date
2015-01-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-84939223945
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.93, No.2 (2015), 241-243
Suggested Citation
Vanaporn Wuthiekanun, Premjit Amornchai, Sayan Langla, Nicholas J. White, Nicholas P.J. Day, Direk Limmathurotsakul, Sharon J. Peacock Antimicrobial disk susceptibility testing of leptospira spp. Using leptospira vanaporn wuthiekanun (LVW) agar. American Journal of Tropical Medicine and Hygiene. Vol.93, No.2 (2015), 241-243. doi:10.4269/ajtmh.15-0180 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/36152
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antimicrobial disk susceptibility testing of leptospira spp. Using leptospira vanaporn wuthiekanun (LVW) agar
Other Contributor(s)
Abstract
© 2015 by The American Society of Tropical Medicine and Hygiene. Leptospira Vanaporn Wuthiekanun (LVW) agar was used to develop a disk diffusion assay for Leptospira spp. Ten pathogenic Leptospira isolates were tested, all of which were susceptible to 17 antimicrobial agents (amoxicillin/ clavulanic acid, amoxicillin, azithromycin, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, doripenem, doxycycline, gentamicin, linezolid, nitrofurantoin, penicillin, piperacillin/tazobactam, and tetracycline). All 10 isolates had no zone of growth inhibition for four antimicrobials (fosfomycin, nalidixic acid, rifampicin, and trimethoprim/ sulfamethoxazole). Of the ten Leptospira, seven had a growth inhibition zone of 21 mm for aztreonam, the zone diameter susceptibility break point for Enterobacteriaceae. This assay could find utility as a simple screening method during the epidemiological surveillance of antimicrobial resistance in Leptospira spp.