Publication: Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression
Issued Date
2015-01-01
Resource Type
ISSN
17507448
1750743X
1750743X
Other identifier(s)
2-s2.0-84942108152
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Immunotherapy. Vol.7, No.7 (2015), 765-775
Suggested Citation
Nattawat Onlamoon, Vajee Petphong, Kasama Sukapirom, Siyu Wang, Palanee Ammaranond, Kovit Pattanapanyasat Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression. Immunotherapy. Vol.7, No.7 (2015), 765-775. doi:10.2217/imt.15.41 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/36157
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Production of anti-CD3/28 expanded CD4<sup>+</sup> T lymphocytes from HIV-infected patients with different degrees of disease progression
Other Contributor(s)
Abstract
© 2015 Future Medicine Ltd. Aims: CD4+ T lymphocytes from HIV-infected patients with different degrees of disease progression based on CD4 count were expanded in vitro using anti-CD3/28-coated beads. Materials & methods: Purified CD4+ T lymphocytes from healthy subjects and patients were expanded for 3 weeks. Moreover, the improvement of cell expansion by IL-2 supplementation was also determined. Results: Expanded CD4+ T lymphocytes from patients had lower fold expansion when compared with healthy subjects. Furthermore, patients with high CD4 counts had higher fold expansion than patients with low CD4 count, and IL-2 supplementation further increased cell expansion. Conclusions: Anti-CD3/28 activation failed to potently induce expansion of CD4+ T lymphocytes from patients. However, the cell expansion could be improved by IL-2 supplementation.