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Sensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reaction

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dc.contributor.authorSuvit Limawongpraneeen_US
dc.contributor.authorDarawan Wanachiwanawinen_US
dc.contributor.authorKulkanya Chokephaibulkiten_US
dc.contributor.authorJerawan Onchrotchanakunen_US
dc.contributor.authorPunpop Lertlaituanen_US
dc.contributor.authorSuphaluck Wankhomen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-08-24T02:01:09Z
dc.date.available2018-08-24T02:01:09Z
dc.date.issued2007-09-01en_US
dc.description.abstractOne hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis jirovecii. Single-step polymerase chain reaction (PCR) produced 13 % (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).en_US
dc.identifier.citationSoutheast Asian Journal of Tropical Medicine and Public Health. Vol.38, No.5 (2007), 892-896en_US
dc.identifier.issn01251562en_US
dc.identifier.other2-s2.0-35349031020en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/24752
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35349031020&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleSensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reactionen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35349031020&origin=inwarden_US

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