Publication: Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
Issued Date
2009-01-01
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ISSN
16788060
00740276
00740276
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2-s2.0-70149093169
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Mahidol University
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SCOPUS
Bibliographic Citation
Memorias do Instituto Oswaldo Cruz. Vol.104, No.4 (2009), 558-566
Suggested Citation
Sorawat Thongsahuan, Visut Baimai, Yasushi Otsuka, Atiporn Saeung, Benjawan Tuetun, Narissara Jariyapan, Sittiporn Suwannamit, Pradya Somboon, Atchariya Jitpakdi, Hiroyuki Takaoka, Wej Choochote Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand. Memorias do Instituto Oswaldo Cruz. Vol.104, No.4 (2009), 558-566. doi:10.1590/S0074-02762009000400004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28264
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Title
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
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Abstract
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2generations, suggest-ing a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies varia-tion (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.