Publication: Nematicidal effect of plumbagin on Caenorhabditis elegans: A model for testing a nematicidal drug
Issued Date
2016-05-01
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ISSN
09395075
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2-s2.0-84974839199
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Mahidol University
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SCOPUS
Bibliographic Citation
Zeitschrift fur Naturforschung - Section C Journal of Biosciences. Vol.71, No.5-6 (2016), 121-131
Suggested Citation
Phantip Chaweeborisuit, Chinnawut Suriyonplengsaeng, Worawit Suphamungmee, Prasert Sobhon, Krai Meemon Nematicidal effect of plumbagin on Caenorhabditis elegans: A model for testing a nematicidal drug. Zeitschrift fur Naturforschung - Section C Journal of Biosciences. Vol.71, No.5-6 (2016), 121-131. doi:10.1515/znc-2015-0222 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43057
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Title
Nematicidal effect of plumbagin on Caenorhabditis elegans: A model for testing a nematicidal drug
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Abstract
© 2016 by De Gruyter 2016. Plumbagin, (5-hydroxy-2-methyl-1,4-naphthoquinone), a natural substance found in the roots of plant species in the genus Plumbago, has been used as a traditional medicine against many diseases. In this study, Caenorhabditis elegans was used as a model for testing the anthelmintic effect of plumbagin. The compound exhibited a nematicidal effect against all stages of C. elegans: L4 was least susceptible, while L1 was most susceptible to plumbagin with an LC50of 220 and 156 μM, respectively. Plumbagin inhibited C. elegans development from L1 to adult stages with an IC50of 235 μM, and body length was also reduced at concentrations of 25 and 50 μg/ml. Brood sizes decreased from 203±6 to 43±6 and 18±3 eggs per hatch in plumbagin-treated worms at 10, 25, 50 μg/ml, respectively. Furthermore, plumbagin was lethal to strains resistant to the nematicides levamisole, albendazole, and ivermectin, indicating that it possesses a strong and unique nematicidal action. Plumbagin decreased the number of mitochondria in hypodermal and intestinal cells and body wall muscles and damaged the ultrastructure of these tissues. Taken together, plumbagin may be a new drug against parasitic nematodes.