Publication: Stability of barakol under hydrolytic stress conditions and its major degradation product
Issued Date
2009-03-01
Resource Type
ISSN
14390221
00320943
00320943
Other identifier(s)
2-s2.0-65649111135
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Planta Medica. Vol.75, No.4 (2009), 346-350
Suggested Citation
Boonrat Chantong, Supim Wongtongtair, Punnee Nusuetrong, Uthai Sotanaphun, Chaiyo Chaichantipyuth, Duangdeun Meksuriyen Stability of barakol under hydrolytic stress conditions and its major degradation product. Planta Medica. Vol.75, No.4 (2009), 346-350. doi:10.1055/s-0028-1112206 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27277
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Stability of barakol under hydrolytic stress conditions and its major degradation product
Other Contributor(s)
Abstract
The aim of the present study was to investigate the stability of barakol, an anxiolytic constituent extracted from leaves of Senna siamea (Lam.) Irwin & Barneby (syn. Cassia siamea Lam.), under the International Conference on Harmonisation suggested conditions using HPLC with photodiode array detection. Extensive degradation of barakol was found to occur under alkaline conditions through base-catalyzed hydrolysis. Mild degradation of barakol was observed under thermal and oxidative stress while it was stable under acidic conditions. The reaction rate constants (kobs) of barakol degradation under alkaline conditions at pHs 12 and 13 were 3.0x10-5 and 9.6x10 -3 min-1, respectively. The activation energy according to the Arrhenius plotwas calculated to be 26.9 ± 3.3 kcal/mol at pH 13 and temperatures between 12 and 51 °C. The major degradation product of barakol under both alkaline and thermal stress conditions was characterized by LC-MS and NMR as cassiachromone. © Georg Thieme Verlag KG Stuttgart.