Publication:
The House Dust Mite Major Allergen der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity

Issued Date

2016-03-01

Resource Type

Article

ISSN

14230097
10182438

DOI

10.1159/000442176

Other identifier(s)

2-s2.0-84973503067

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

International Archives of Allergy and Immunology. Vol.168, No.3 (2016), 150-160

Suggested Citation

Wai Tuck Soh, Maxime Le Mignon, Narissara Suratannon, Pattraporn Satitsuksanoa, Pantipa Chatchatee, Jongkonnee Wongpiyaboron, Mukda Vangveravong, Ticha Rerkpattanapipat, Atik Sangasapaviliya, Emmanuel Nony, Surapon Piboonpocanun, Kiat Ruxrungtham, Alain Jacquet, Pattarawat Thantiworasit, Pinya Pulsawat, Tassalalpa Daengsuwan, Wannada Laisuan, Malinee Tongdee, Nizchapha Dchapaphapeaktak, Tadech Boonpiyathad The House Dust Mite Major Allergen der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity. International Archives of Allergy and Immunology. Vol.168, No.3 (2016), 150-160. doi:10.1159/000442176 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40867

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Title

The House Dust Mite Major Allergen der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity

Author(s)

Wai Tuck Soh
 Maxime Le Mignon
 Narissara Suratannon
 Pattraporn Satitsuksanoa
 Pantipa Chatchatee
 Jongkonnee Wongpiyaboron
 Mukda Vangveravong
 Ticha Rerkpattanapipat
 Atik Sangasapaviliya
 Emmanuel Nony
 Surapon Piboonpocanun
 Kiat Ruxrungtham
 Alain Jacquet
 Pattarawat Thantiworasit
 Pinya Pulsawat
 Tassalalpa Daengsuwan
 Wannada Laisuan
 Malinee Tongdee
 Nizchapha Dchapaphapeaktak
 Tadech Boonpiyathad

Other Contributor(s)

Chulalongkorn University
 Division of Allergy and Immunology
 Queen Sirikit National Institute of Child Health
 Mahidol University
 Phramongkutklao College of Medicine
 Stallergenes
 King Chulalongkorn Memorial Hospital, Faculty of Medicine Chulalongkorn University
 Children Hospital
 Faculty of Medicine, Ramathibodi Hospital, Mahidol University

Abstract

© 2016 S. Karger AG, Basel. Background: The in-depth characterization of the recently identified house dust mite (HDM) major allergen Der p 23 requires the production of its recombinant counterpart because the natural allergen is poorly extractable from fecal pellets. This study aimed to provide a detailed physico-chemical characterization of recombinant Der p 23 (rDer p 23) as well as to investigate its IgE reactivity in a cohort of HDM-allergic patients from Thailand. Methods: Purified rDer p 23, secreted from recombinant Pichia pastoris, was characterized by mass spectrometry and circular dichroism analyses as well as for its chitin-binding activity. The IgE-binding frequency and allergenicity of Der p 23 were determined by ELISA and RBL-SX38 degranulation assays, respectively. Results: Purified intact rDer p 23 carried O-mannosylation and mainly adopted a random coil structure. Polyclonal antibodies to rDer p 23 can detect the corresponding natural allergen (nDer p 23) in aqueous fecal pellet extracts, suggesting that both forms of Der p 23 share common B-cell epitopes. Despite its homologies with chitin-binding proteins, both natural Der p 23 and rDer p 23 were unable to interact in vitro with chitin matrices. Of 222 Thai HDM-allergic patients tested, 54% displayed Der p 23-specific IgE responses. Finally, the allergenicity of rDer p 23 was confirmed by the degranulation of rat basophil leukemia cells. Conclusion: Our findings highlighted important levels of Der p 23 sensitizations in Thailand. Our study clearly suggested that rDer p 23 is likely more appropriate for HDM allergy component-resolved diagnosis than HDM extracts.

Keyword(s)

Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/40867

Collections

Scopus 2016-2017