Publication: Detection of Opisthorchis viverrini antigens in stools using specific monoclonal antibody
Issued Date
1992-01-01
Resource Type
ISSN
00207519
Other identifier(s)
2-s2.0-0026680867
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.22, No.4 (1992), 527-531
Suggested Citation
W. Chaicumpa, L. Ybanez, V. Kitikoon, S. Pungpak, Y. Ruangkunaporn, M. Chongsa-Nguan, S. Sornmani Detection of Opisthorchis viverrini antigens in stools using specific monoclonal antibody. International Journal for Parasitology. Vol.22, No.4 (1992), 527-531. doi:10.1016/0020-7519(92)90155-E Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22296
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Title
Detection of Opisthorchis viverrini antigens in stools using specific monoclonal antibody
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Abstract
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detecting Opisthorchis viverrini antigen in faecal extracts of four groups of individuals. These were 24 patients with O. viverrini infection only (group 1), 31 patients with O. viverrini and other parasitic infections (group 2), 141 patients with other parastitic infections (group 3) and 21 normal, parasite-free individuals (group 4). The first antibody used in the ELISA was polyclonal immunoglobulin G prepared from the serum of a rabbit previously immunized with crude extract of O. viverrini. The second antibody was monoclonal antibody specific to an antigen located in the worm tegument and muscular tissue. Sensitivity of the assay was 31% while specificity was 100%. Considerations for improving the sensitivity are discussed. © 1992.