Publication: Clostridium manihotivorum sp. nov., a novel mesophilic anaerobic bacterium that produces cassava pulp-degrading enzymes
Issued Date
2020-11-16
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ISSN
21678359
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2-s2.0-85096295727
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Mahidol University
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SCOPUS
Bibliographic Citation
PeerJ. Vol.8, (2020)
Suggested Citation
Pattsarun Cheawchanlertfa, Sawannee Sutheeworapong, Piroon Jenjaroenpun, Thidathip Wongsurawat, Intawat Nookaew, Supapon Cheevadhanarak, Akihiko Kosugi, Patthra Pason, Rattiya Waeonukul, Khanok Ratanakhanokchai, Chakrit Tachaapaikoon Clostridium manihotivorum sp. nov., a novel mesophilic anaerobic bacterium that produces cassava pulp-degrading enzymes. PeerJ. Vol.8, (2020). doi:10.7717/peerj.10343 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/60347
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Title
Clostridium manihotivorum sp. nov., a novel mesophilic anaerobic bacterium that produces cassava pulp-degrading enzymes
Abstract
Copyright 2020 Cheawchanlertfa et al. Distributed under Creative Commons CC-BY 4.0 Background. Cassava pulp is a promising starch-based biomasses, which consists of residual starch granules entrapped in plant cell wall containing non-starch polysaccharides, cellulose and hemicellulose. Strain CT4T, a novel mesophilic anaerobic bacterium isolated from soil collected from a cassava pulp landfill, has a strong ability to degrade polysaccharides in cassava pulp. This study explored a rarely described species within the genus Clostridium that possessed a group of cassava pulp-degrading enzymes. Methods. A novel mesophilic anaerobic bacterium, the strain CT4T, was identified based on phylogenetic, genomic, phenotypic and chemotaxonomic analysis. The complete genome of the strain CT4T was obtained following whole-genome sequencing, assembly and annotation using both Illumina and Oxford Nanopore Technology (ONT) platforms. Results. Analysis based on the 16S rRNA gene sequence indicated that strain CT4T is a species of genus Clostridium. Analysis of the whole-genome average amino acid identity (AAI) of strain CT4T and the other 665 closely related species of the genus Clostridium revealed a separated strain CT4T from the others. The results revealed that the genome consisted of a 6.3 Mb circular chromosome with 5,664 protein-coding sequences. Genome analysis result of strain CT4T revealed that it contained a set of genes encoding amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. A comparative genomic analysis of strain CT4T with closely related species with available genomic information, C. amylolyticum SW408T, showed that strain CT4T contained more genes encoding cassava pulp-degrading enzymes, which comprised a complex mixture of amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes.