Publication:
The indirect immunofluorescence assay using cardiac tissue from chickens, quails and ducks for identification of influenza A virus during an outbreak of highly pathogenic avian influenza virus (H5N1): A rapid and simple screening tool for limited resource settings

Issued Date

2007-10-01

Resource Type

Article

ISSN

00345288

DOI

10.1016/j.rvsc.2006.12.007

Other identifier(s)

2-s2.0-34347250844

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Research in Veterinary Science. Vol.83, No.2 (2007), 279-281

Suggested Citation

Chongmas Antarasena, Rungtiva Sirimujalin, Porntip Prommuang, Naruepol Promkuntod, Praison Prommuang, Stuart D. Blacksell The indirect immunofluorescence assay using cardiac tissue from chickens, quails and ducks for identification of influenza A virus during an outbreak of highly pathogenic avian influenza virus (H5N1): A rapid and simple screening tool for limited resource settings. Research in Veterinary Science. Vol.83, No.2 (2007), 279-281. doi:10.1016/j.rvsc.2006.12.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23974

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Title

The indirect immunofluorescence assay using cardiac tissue from chickens, quails and ducks for identification of influenza A virus during an outbreak of highly pathogenic avian influenza virus (H5N1): A rapid and simple screening tool for limited resource settings

Author(s)

Chongmas Antarasena
 Rungtiva Sirimujalin
 Porntip Prommuang
 Naruepol Promkuntod
 Praison Prommuang
 Stuart D. Blacksell

Other Contributor(s)

Southern Veterinary Research and Development Center
 Mahidol University
 Churchill Hospital

Abstract

Here we describe the diagnostic utility of the indirect immunofluorescence assay (IFA) during a recent outbreak of highly pathogenic avian influenza (HPAI) subtype H5N1 virus in southern Thailand and demonstrate the usefulness of the cardiac tissue from infected chickens, quail, and ducks for diagnosis. The most reliable sample for IFA diagnosis of influenza A virus was cardiac tissue (83.0%; 44/53) which when divided by species (chicken, quail and duck cardiac tissues) gave respective positivity rates of 88% (22/25), 88.9% (16/18) and 60.0% (6/10). Cardiac tissue also gave the highest IFA intensity for the three species. We believe that the IFA method has wide applicability in developing countries or remote settings where clinically similar avian diseases with high morbidity and mortality such as Newcastle disease and fowl cholera are common and could be rapidly excluded thereby conserving valuable reference laboratory capacity for true HPAI outbreaks. © 2007 Elsevier Ltd. All rights reserved.

Keyword(s)

Agricultural and Biological Sciences
 Veterinary

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/23974

Collections

Scopus 2006-2010