Publication: DNA topoisomerase IIα inhibitory and anti-HIV-1 flavones from leaves and twigs of Gardenia carinata
Issued Date
2012-03-01
Resource Type
ISSN
18736971
0367326X
0367326X
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2-s2.0-84855985112
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Mahidol University
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SCOPUS
Bibliographic Citation
Fitoterapia. Vol.83, No.2 (2012), 368-372
Suggested Citation
Naowarat Kongkum, Patoomratana Tuchinda, Manat Pohmakotr, Vichai Reutrakul, Pawinee Piyachaturawat, Surawat Jariyawat, Kanoknetr Suksen, Chalobon Yoosook, Jitra Kasisit, Chanita Napaswad DNA topoisomerase IIα inhibitory and anti-HIV-1 flavones from leaves and twigs of Gardenia carinata. Fitoterapia. Vol.83, No.2 (2012), 368-372. doi:10.1016/j.fitote.2011.11.015 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15170
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Title
DNA topoisomerase IIα inhibitory and anti-HIV-1 flavones from leaves and twigs of Gardenia carinata
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Abstract
Four new flavones, 5,2′-dihydroxy-7,3′,4′,5′- tetramethoxyflavone (1), 5,2′,5′-trihydroxy-7,3′,4′- trimethoxyflavone (2), 5,7,2′,5′-tetrahydroxy-6,3′,4′- trimethoxyflavone (3) and 5,2′,5′-trihydroxy-6,7,3′,4′- tetramethoxyflavone (4), along with the known 5,3′-dihydroxy-6,7,4′, 5′-tetramethoxyflavone (5), 5,7,3′,5′-tetrahydroxy-6,4′- dimethoxyflavone (6), syringaldehyde, vanillic acid and scopoletin were isolated from the leaves and twigs of Gardenia carinata (Rubiaceae). Their structures were determined by spectroscopic methods. Flavone 2 exhibited cytotoxic activity against P-388 and MCF-7 cell lines, while 3, 5 and 6 were active only in P-388 cell line. All active compounds were found to inhibit DNA topoisomerase IIα activity, which may be responsible for the observed cytotoxicity. Flavones 1-3, 5 and 6 also exhibited anti-HIV-1 activity in the anti-syncytium assay using δTat/rev MC99 virus and 1A2 cell line system; 2 was most potent. Only flavones 1 and 6 showed considerably activity against HIV-1 reverse transcriptase. © 2011 Elsevier B.V. All rights reserve.