Publication: Simultaneous Determination of Artemether and Azithromycin in Suppositories by Reversed Phase HPLC
Issued Date
2011-11-01
Resource Type
ISSN
1532236X
00032719
00032719
Other identifier(s)
2-s2.0-84857770029
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Letters. Vol.44, No.16 (2011), 2732-2743
Suggested Citation
Karen Gaudin, Tina Kauss, Alexandra Gaubert, Vincent Viaud, Jean Pierre Dubost, Piero Olliaro, Nicholas J. White, Pascal Millet Simultaneous Determination of Artemether and Azithromycin in Suppositories by Reversed Phase HPLC. Analytical Letters. Vol.44, No.16 (2011), 2732-2743. doi:10.1080/00032719.2011.553014 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11448
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Title
Simultaneous Determination of Artemether and Azithromycin in Suppositories by Reversed Phase HPLC
Abstract
Chromatographic parameter assessments for RP-HPLC-UV method development for the simultaneous analysis of artemether and azithromycin for the pharmaceutical analysis of a rectal coformulation currently under development for the treatment of malaria infected children. Using methanol based mobile phase for the analysis of both artemether and azithromycin provided a more robust method in terms of resolution and peak symmetry. The method validated for suppository used 80% methanol and 20% phosphate buffer 15 mM at pH 9. The UV detection was at 210 nm. The accuracy profiles indicated a method validation between 80-120% for both active pharmaceutical ingredients. The preparation process of the suppository was validated based on theoretical values of artemether and azithromycin present in the formulation; active pharmaceutical ingredients were homogenously distributed within the suppository. © 2011 Copyright Taylor and Francis Group, LLC.