Publication: ChpR is a chlorpyrifos-responsive transcription regulator in Sinorhizobium meliloti
Issued Date
2010-06-01
Resource Type
ISSN
14641801
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2-s2.0-77950625467
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Molecular Microbiology and Biotechnology. Vol.18, No.3 (2010), 141-147
Suggested Citation
Wirongrong Whangsuk, James M. Dubbs, Ratiboot Sallabhan, Kumpanart Somsongkul, Skorn Mongkolsuk, Suvit Loprasert ChpR is a chlorpyrifos-responsive transcription regulator in Sinorhizobium meliloti. Journal of Molecular Microbiology and Biotechnology. Vol.18, No.3 (2010), 141-147. doi:10.1159/000308514 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28698
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Title
ChpR is a chlorpyrifos-responsive transcription regulator in Sinorhizobium meliloti
Abstract
The broad-spectrum organophosphate insecticide chlorpyrifos (CPF)-inducible locus, chpAB, was identified on the endogenous plasmid pSymB in Sinorhizobium meliloti. The S. meliloti chpA promoter was highly induced by CPF and was induced at much lower levels by diazinon and ethion. Transcription of chpA was dependent on chpR, a CadC family transcriptional regulator located upstream of, and divergently transcribed from, chpAB. ChpR was able to mediate the CPF-inducible expression of the S. melilotichpA promoter in Escherichia coli through direct interaction with the chpAB promoter. The chpR-chpA intergenic regions of several bacterial chpRAB operons were aligned and a putative ChpR-binding sequence was proposed. Both the ChpR transcription factor and chpA promoter constitute a good candidate system for genetic-based biosensor development. © 2010 S. Karger AG, Basel.