Publication:
Inhibition of N-myristoyltransferase1 affects dengue virus replication

dc.contributor.authorSan Suwanmaneeen_US
dc.contributor.authorYuvadee Mahakhunkijcharoenen_US
dc.contributor.authorSumate Ampawongen_US
dc.contributor.authorPornsawan Leaungwutiwongen_US
dc.contributor.authorDorothée Misséen_US
dc.contributor.authorNatthanej Luplertlopen_US
dc.contributor.otherMaladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôleen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2020-01-27T08:54:15Z
dc.date.available2020-01-27T08:54:15Z
dc.date.issued2019-09-01en_US
dc.description.abstract© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. Dengue virus (DENV) causes dengue fever, a self-limiting disease that could be fatal due to serious complications. No specific treatment is currently available and the preventative vaccine is only partially protective. To develop a potential drug target for dengue fever, we need to understand its biology and pathogenesis thoroughly. N-myristoyltransferase (NMT) is an N-terminal protein lipidation enzyme that catalyzes the covalent cotranslational attachment of fatty acids to the amino-terminal glycine residue of a number of proteins, leading to the modulation of various signaling molecules. In this study, we investigated the interaction of dengue viral proteins with host NMT and its subsequent effect on DENV. Our bioinformatics, molecular docking, and far-western blotting analyses demonstrated the interaction of viral envelope protein (E) with NMT. The gene expression of NMT was strongly elevated in a dependent manner during the viral replication phase in dendritic cells. Moreover, NMT gene silencing significantly inhibited DENV replication in dendritic cells. Further studies investigating the target cell types of other host factors are suggested.en_US
dc.identifier.citationMicrobiologyOpen. Vol.8, No.9 (2019)en_US
dc.identifier.doi10.1002/mbo3.831en_US
dc.identifier.issn20458827en_US
dc.identifier.other2-s2.0-85062698294en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/51024
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062698294&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleInhibition of N-myristoyltransferase1 affects dengue virus replicationen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062698294&origin=inwarden_US

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