Plant Cell, Tissue and Organ Culture. Vol.80, No.3 (2005), 347-351
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S. Prathanturarug, N. Soonthornchareonnon, W. Chuakul, Y. Phaidee, P. Saralamp Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium. Plant Cell, Tissue and Organ Culture. Vol.80, No.3 (2005), 347-351. doi:10.1007/s11240-004-1020-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16220
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Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production.
