Publication: Curcumin inhibited hepatitis B viral entry through NTCP binding
Issued Date
2021-12-01
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ISSN
20452322
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2-s2.0-85115768354
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Mahidol University
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SCOPUS
Bibliographic Citation
Scientific Reports. Vol.11, No.1 (2021)
Suggested Citation
Piyanoot Thongsri, Yongyut Pewkliang, Suparerk Borwornpinyo, Adisak Wongkajornsilp, Suradej Hongeng, Khanit Sa-ngiamsuntorn Curcumin inhibited hepatitis B viral entry through NTCP binding. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-98243-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79212
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Title
Curcumin inhibited hepatitis B viral entry through NTCP binding
Abstract
Hepatitis B virus (HBV) has been implicated in hepatitis and hepatocellular carcinoma. Current agents (nucleos(t)ide analogs and interferons) could only attenuate HBV infection. A combination of agents targeting different stages of viral life cycle (e.g., entry, replication, and cccDNA stability) was expected to eradicate the infection. Curcumin (CCM) was investigated for inhibitory action toward HBV attachment and internalization. Immortalized hepatocyte-like cells (imHCs), HepaRG and non-hepatic cells served as host cells for binding study with CCM. CCM decreased viral load, HBeAg, HBcAg (infectivity), intracellular HBV DNA, and cccDNA levels. The CCM-induced suppression of HBV entry was directly correlated with the density of sodium-taurocholate co-transporting polypeptide (NTCP), a known host receptor for HBV entry. The site of action of CCM was confirmed using TCA uptake assay. The affinity between CCM and NTCP was measured using isothermal titration calorimetry (ITC). These results demonstrated that CCM interrupted HBV entry and would therefore suppress HBV re-infection.