Publication: Combined gold nanoparticle-antibody conjugate enhances sensitivity of influenza a antigen detection in lateral flow assay
Issued Date
2012-12-01
Resource Type
ISSN
21596972
21596964
21596964
Other identifier(s)
2-s2.0-84880212034
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Mahidol University
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SCOPUS
Bibliographic Citation
IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. (2012), 36-40
Suggested Citation
Natpapas Wiriyachaiporn, Nisachon Apiwat, Warangkana Chantima, Tararaj Dharakul Combined gold nanoparticle-antibody conjugate enhances sensitivity of influenza a antigen detection in lateral flow assay. IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. (2012), 36-40. doi:10.1109/NANOMED.2012.6509123 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13568
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Title
Combined gold nanoparticle-antibody conjugate enhances sensitivity of influenza a antigen detection in lateral flow assay
Abstract
A combined gold nanoparticle (GNP)-monoclonal antibody (MAb) conjugate was generated for a direct influenza A antigen detection using lateral flow assay (LF) format. The combination of GNP-MAb conjugate targeting both nucleoprotein (NP) and matrix protein (M) allows the detection of multiple influenza A proteins. The LF system consisting of combined GNP-MAb conjugate showed an improved sensitivity, compared to the LF system containing single GNP-MAb conjugate targeting only one target protein, either NP or M. This was illustrated by the 2- to 260-fold lower limit of detection of the combined GNP-MAb conjugate LF than the single GNP-MAb conjugate LF. As such, the combined GNP-MAb conjugate LF could provide the basis for developing a direct influenza A antigen detection for rapid influenza A diagnosis. © 2012 IEEE.