Publication: Intratypic variations among Thai Herpes Simplex Virus (HSV) isolates determined by Restriction Fragment Length Polymorphism (RFLP) analysis
Issued Date
2005-07-01
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ISSN
01251562
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2-s2.0-27744450595
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.4 (2005), 910-916
Suggested Citation
Sutida Visaprom, Ariya Chindamporn, Wasun Chantratita, Parvapan Bhattarakosol Intratypic variations among Thai Herpes Simplex Virus (HSV) isolates determined by Restriction Fragment Length Polymorphism (RFLP) analysis. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.4 (2005), 910-916. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16920
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Title
Intratypic variations among Thai Herpes Simplex Virus (HSV) isolates determined by Restriction Fragment Length Polymorphism (RFLP) analysis
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Abstract
Whole genomic polymorphisms for 20 HSV-1 and 20 HSV-2 isolates from Thai patients were analyzed by means of Restriction Fragment Length Polymorphism (RFLP) analysis using 4 restriction endonucleases: BamHI, KpnI, HindIII, and EcoRI. Variations in cleavage sites among the HSV-1 and HSV-2 isolates were compared to the cleavage patterns of standard HSV-1 strain KOS and HSV-2 strain Baylor 186. Although 70% of HSV-1 isolates with BamHI digestion, 50% with KpnI, 75% with HindIII and 70% with EcoRI digestion were found to be similar to the standard HSV-1 (KOS) pattern, new BamHI restriction sites were detected in some HSV-1 isolates. For HSV-2 isolates, 85% had the same pattern as the standard HSV-2 (Baylor 186) after digestion with BamHI, HindIII, and EcoRI. No difference was observed with KpnI digestion. When the patterns from the 4 enzymes were combined, HSV-1 isolates showed more divergence than the HSV-2 isolates. HSV-1 isolates found in both non-genital and genital lesions had more variety than the HSV-2 isolates. This suggests that intratypic variations in HSV-2 are fewer than in HSV-1.