Publication: Structural and antigenic analysis of the yellow head virus nucleocapsid protein p20
Issued Date
2006-03-01
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ISSN
01681702
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2-s2.0-32344436343
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Mahidol University
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SCOPUS
Bibliographic Citation
Virus Research. Vol.116, No.1-2 (2006), 21-29
Suggested Citation
Nusra Sittidilokratna, Natthida Phetchampai, Vichai Boonsaeng, Peter J. Walker Structural and antigenic analysis of the yellow head virus nucleocapsid protein p20. Virus Research. Vol.116, No.1-2 (2006), 21-29. doi:10.1016/j.virusres.2005.08.009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23076
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Title
Structural and antigenic analysis of the yellow head virus nucleocapsid protein p20
Abstract
Yellow head virus (YHV) is an invertebrate nidovirus that is highly pathogenic for marine shrimp. Nucleotide sequence analysis indicated that the YHV ORF2 gene encodes a basic protein (pI = 9.9) of 146 amino acids with a predicted molecular weight of 16,325.5 Da. The deduced amino acid sequence indicated a predominance of basic (15.1%), acidic (9.6%) and hydrophilic polar (34.3%) residues and a high proportion proline and glycine residues (16.4%). The ORF2 gene was cloned and expressed in Escherichia coli as a Mr= 21 kDa His6-protein that reacted with YHV nucleoprotein (p20) monoclonal antibody. Segments representing the four linear quadrants of the nucleoprotein were also expressed in E. coli as GST-fusion proteins. Immunoblot analysis using YHV polyclonal rabbit antiserum indicated the presence of linear epitopes in all except the V37-Q74quadrant. Immunoblot analysis of the GST-fusion proteins and C-terminally truncated segments of the nucleoprotein allowed mapping of YHV monoclonal antibodies Y19, Y20 and YII4 to linear epitopes in the acidic domain between amino acids I116and E137. The full-length nucleoprotein was expressed at high level in E. coli and was easily purified in quantity from the soluble cell fraction by Ni+-NTA affinity chromatography. © 2005 Elsevier B.V. All rights reserved.