Publication: Protective effect of panduratin a on cisplatin-induced apoptosis of human renal proximal tubular cells and acute kidney injury in mice
dc.contributor.author | Penjai Thongnuanjan | en_US |
dc.contributor.author | Sirima Soodvilai | en_US |
dc.contributor.author | Somsak Fongsupa | en_US |
dc.contributor.author | Napason Chabang | en_US |
dc.contributor.author | Pornpun Vivithanaporn | en_US |
dc.contributor.author | Patoomratana Tuchinda | en_US |
dc.contributor.author | Sunhapas Soodvilai | en_US |
dc.contributor.other | Rangsit University | en_US |
dc.contributor.other | Faculty of Medicine Ramathibodi Hospital, Mahidol University | en_US |
dc.contributor.other | Mahidol University | en_US |
dc.contributor.other | Thammasat University | en_US |
dc.date.accessioned | 2022-08-04T11:21:50Z | |
dc.date.available | 2022-08-04T11:21:50Z | |
dc.date.issued | 2021-06-01 | en_US |
dc.description.abstract | Background: Cisplatin is an effective chemotherapy but its main side effect, acute kidney injury, limits its use. Panduratin A, a bioactive compound extracted from Boesenbergia rotunda, shows several biological activities such as anti-oxidative effects. The present study investigated the nephroprotective effect of panduratin A on cisplatin-induced renal injury. Methods: We investigated the effect of panduratin A on the toxicity of cisplatin in both mice and human renal cell cultures using RPTEC/TERT1 cells. Results: The results demonstrated that panduratin A ameliorates cisplatin-induced renal toxicity in both mice and RPTEC/ TERT1 cells by reducing apoptosis. Mice treated with a single intraperitoneal (i.p.) injection of cisplatin (20mg/kg body weight (BW)) exhibited renal tubule injury and impaired kidney function as shown by histological examination and increased serum creatinine. Co-administration of panduratin A (50mg/kg BW) orally improved kidney function and ameliorated renal tubule injury of cisplatin by inhibiting activation of extracellular signal-regulated kinase (ERK)1/2 and caspase 3. In human renal proximal tubular cells, cisplatin induced cell apoptosis by activating pro-apoptotic proteins (ERK1/2 and caspase 3), and reducing the anti-apoptotic protein (Bcl-2). These effects were significantly ameliorated by co-treatment with panduratin A. Interestingly, panduratin A did not alter intracellular accumulation of cisplatin. It did not alter the anticancer efficacy of cisplatin in either human colon or non-small cell lung cancer cell lines. Conclusions: The present study highlights panduratin A has a potential protective effect on cisplatin’s nephrotoxicity. | en_US |
dc.identifier.citation | Biological and Pharmaceutical Bulletin. Vol.44, No.6 (2021), 830-837 | en_US |
dc.identifier.doi | 10.1248/bpb.b21-00036 | en_US |
dc.identifier.issn | 13475215 | en_US |
dc.identifier.issn | 09186158 | en_US |
dc.identifier.other | 2-s2.0-85107194643 | en_US |
dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/123456789/78959 | |
dc.rights | Mahidol University | en_US |
dc.rights.holder | SCOPUS | en_US |
dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107194643&origin=inward | en_US |
dc.subject | Pharmacology, Toxicology and Pharmaceutics | en_US |
dc.title | Protective effect of panduratin a on cisplatin-induced apoptosis of human renal proximal tubular cells and acute kidney injury in mice | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107194643&origin=inward | en_US |