Publication: Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection
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Issued Date
2020-10-16
Resource Type
ISSN
18734324
00032670
00032670
Other identifier(s)
2-s2.0-85089810860
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Mahidol University
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SCOPUS
Bibliographic Citation
Analytica Chimica Acta. Vol.1134, (2020), 10-17
Suggested Citation
Tatchanun Ngamdee, Lee Su Yin, Sompong Vongpunsawad, Yong Poovorawan, Werasak Surareungchai, Benchaporn Lertanantawong Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection. Analytica Chimica Acta. Vol.1134, (2020), 10-17. doi:10.1016/j.aca.2020.08.018 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/58945
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Title
Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection
Abstract
© 2020 Elsevier B.V. DNA strand displacement is an attractive, enzyme-free target hybridization strategy for nano-biosensing. The target DNA induces a strand displacement reaction by replacing the pre-hybridized strand that is labeled with gold nanoparticles (AuNPs). Thus, the amount of displaced-AuNP-labeled strand is proportional to the amount of target DNA in the sample. The use of a magnetogenosensing technique to isolate the target DNA allows for a simple, one-pot detection approach, which minimizes possible carry-over contamination and pipetting errors. We sought a proof-of-concept for this technology in its ability to detect DNA-equivalent of hepatitis E virus (HEV), which causes acute viral hepatitis for which rapid and simple diagnostic methods remain limited. Signal detection was done via visual observation, spectrophotometry, and electrochemistry. The sensor demonstrated good sensitivity with detection limits of 10 pM (visual), 10 pM (spectrophotometry) and 1 fM (electrochemical). This sensor also exhibited high specificity for real target amplicons and could discriminate between perfect and mismatched sequences. Lyophilized biosensor reagents stored at 4 °C, 25 °C, and outdoor ambient temperature, were stable for up to 90, 50, and 40 days, respectively. The integration of magnetic separation and target DNA-induced strand displacement reaction in a dry reagent form makes the sensing platform easy-to-use and suitable for field settings.