Publication: Oxidative DNA damage and repair in children exposed to low levels of arsenic in utero and during early childhood: Application of salivary and urinary biomarkers
Issued Date
2013-12-15
Resource Type
ISSN
10960333
0041008X
0041008X
Other identifier(s)
2-s2.0-84888879926
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Mahidol University
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SCOPUS
Bibliographic Citation
Toxicology and Applied Pharmacology. Vol.273, No.3 (2013), 569-579
Suggested Citation
Pantip Hinhumpatch, Panida Navasumrit, Krittinee Chaisatra, Jeerawan Promvijit, Chulabhorn Mahidol, Mathuros Ruchirawat Oxidative DNA damage and repair in children exposed to low levels of arsenic in utero and during early childhood: Application of salivary and urinary biomarkers. Toxicology and Applied Pharmacology. Vol.273, No.3 (2013), 569-579. doi:10.1016/j.taap.2013.10.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32724
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Title
Oxidative DNA damage and repair in children exposed to low levels of arsenic in utero and during early childhood: Application of salivary and urinary biomarkers
Abstract
The present study aimed to assess arsenic exposure and its effect on oxidative DNA damage and repair in young children exposed in utero and continued to live in arsenic-contaminated areas. To address the need for biological specimens that can be acquired with minimal discomfort to children, we used non-invasive urinary and salivary-based assays for assessing arsenic exposure and early biological effects that have potentially serious health implications. Levels of arsenic in nails showed the greatest magnitude of difference between exposed and control groups, followed by arsenic concentrations in saliva and urine. Arsenic levels in saliva showed significant positive correlations with other biomarkers of arsenic exposure, including arsenic accumulation in nails (r= 0.56, P<. 0.001) and arsenic concentration in urine (r= 0.50, P<. 0.05). Exposed children had a significant reduction in arsenic methylation capacity indicated by decreased primary methylation index and secondary methylation index in both urine and saliva samples. Levels of salivary 8-OHdG in exposed children were significantly higher (~. 4-fold, P<. 0.01), whereas levels of urinary 8-OHdG excretion and salivary hOGG1 expression were significantly lower in exposed children (~. 3-fold, P<. 0.05), suggesting a defect in hOGG1 that resulted in ineffective cleavage of 8-OHdG. Multiple regression analysis results showed that levels of inorganic arsenic (iAs) in saliva and urine had a significant positive association with salivary 8-OHdG and a significant negative association with salivary hOGG1 expression.© 2013 Elsevier Inc.