Publication: Monoclonal antibodies to LipL32 protect against heterologous Leptospira spp. challenge
Issued Date
2008-12-01
Resource Type
ISSN
15540014
Other identifier(s)
2-s2.0-58149123710
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Mahidol University
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SCOPUS
Bibliographic Citation
Hybridoma. Vol.27, No.6 (2008), 453-465
Suggested Citation
Santi Maneewatch, Yuwaporn Sakolvaree, Patcharin Saengjaruk, Potjanee Srimanote, Pramuan Tapchaisri, Pongsri Tongtawe, Buppa Klaysing, Surasakdi Wongratanacheewin, Manas Chongsa-Nguan, Wanpen Chaicumpa Monoclonal antibodies to LipL32 protect against heterologous Leptospira spp. challenge. Hybridoma. Vol.27, No.6 (2008), 453-465. doi:10.1089/hyb.2008.0056 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19268
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Title
Monoclonal antibodies to LipL32 protect against heterologous Leptospira spp. challenge
Abstract
A non-culture-based leptospirosis vaccine that cross-protects against infection caused by heterologous Leptospira spp. should replace the currently available products, which are qualitatively and quantitatively inadequate. With that in mind, two murine hybridomas secreting monoclonal antibodies (MAb) binding only to homogenates of pathogenic Leptospira spp., and not of the saprophytic L. biflexa, serogroup Patoc, serovar Patoc, were produced. The MAbs of both clones neutralized Leptospira-mediated human red blood cell lysis in vitro and rescued hamsters from lethal infection with heterologous Leptospira spp. The orthologous Leptospira spp. protein carrying the MAb epitope(s) was identified by two-dimensional gel electrophoresis (2DE)-based proteomics and database search. The epitopes of the MAbs were located on the major outer membrane protein LipL32 of the pathogenic Leptospira spp. The MAbs in their humanized version are potential leptospirosis immunotherapeutics. They are also suitable as detection reagents in antigen-based assays for the rapid diagnosis of leptospirosis. Recombinant LipL32 is a good candidate for a broad spectrum, non-culture-based leptospirosis vaccine. © Copyright 2008, Mary Ann Liebert, Inc.