Publication: Simple and fast analysis of iohexol in human serums using micro-hydrophilic interaction liquid chromatography with monolithic column
Issued Date
2016-09-01
Resource Type
ISSN
16159314
16159306
16159306
Other identifier(s)
2-s2.0-84988380509
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Separation Science. Vol.39, No.18 (2016), 3521-3527
Suggested Citation
Thotsaphorn Chaloemsuwiwattanakan, Areeporn Sangcakul, Chagriya Kitiyakara, Duangjai Nacapricha, Prapin Wilairat, Patcharin Chaisuwan Simple and fast analysis of iohexol in human serums using micro-hydrophilic interaction liquid chromatography with monolithic column. Journal of Separation Science. Vol.39, No.18 (2016), 3521-3527. doi:10.1002/jssc.201600475 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43350
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Title
Simple and fast analysis of iohexol in human serums using micro-hydrophilic interaction liquid chromatography with monolithic column
Abstract
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim A simple and rapid method based on micro-liquid chromatography using a synthetic monolithic capillary column was developed for determination of iohexol in human serums, a marker to evaluate the glomerular filtration rate. A hydrophilic methacrylic acid-ethylene dimethacrylate monolith provided excellent selectivity and efficiency for iohexol with separation time of 3 min using a mobile phase of 40:60 v/v 50 mM phosphate buffer pH 5/methanol. Four serum protein removal, methods using perchloric acid, 50% acetonitrile, 0.1 M zinc sulfate, and centrifuge membrane filter were examined. The method of zinc sulfate was chosen due to its simplicity, compatibility with the mobile phase system, nontoxicity, and low cost. Interday calibration curves were conducted over iohexol concentrations range of 2–500 mg/L (R2= 0.9997 ± 0.0001) with detection limit of 0.44 mg/L. Intra- and interday precisions for peak area and retention time were less than 2.8 and 1.4%, respectively. The method was successfully applied to serum samples with percent recoveries from 102 to 104. The method was applied to monitor released iohexol from healthy subject. Compared with the commercially available reversed-phase high-performance liquid chromatography method, the presented method provided simpler chromatogram, faster separation with higher separation efficiency and much lower sample and solvent consumption.