Publication:
Use of a rapid test to assess plasma Plasmodium falciparum HRP2 and guide management of severe febrile illness

dc.contributor.authorIpsita Sinhaen_US
dc.contributor.authorNattwut Ekapiraten_US
dc.contributor.authorArjen M. Dondorpen_US
dc.contributor.authorCharles J. Woodrowen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of Oxforden_US
dc.date.accessioned2018-11-23T10:15:34Z
dc.date.available2018-11-23T10:15:34Z
dc.date.issued2015-09-22en_US
dc.description.abstract© 2015 Sinha et al. Background: Plasma Plasmodium falciparum histidine-rich protein-2 (PfHRP2) is the most accurate biomarker for severe malaria, but its measurement by ELISA has been considered too unwieldy to incorporate into clinical management. Methods: Plasma samples covering a wide range of PfHRP2 concentrations were applied to rapid diagnostic tests (RDTs). RDTs were read by eye and digital capture, and PfHRP2 concentrations were measured via serial dilution with results compared to ELISA readings. Results: The Paracheck <sup>®</sup> brand showed the strongest correlation (r<sup>2</sup> = 0.963) as well as the lowest inter-observer variability (combined kappa across band intensities for three observers = 0.938). Plasma PfHRP2 measurement via serial dilution showed minimal bias compared to ELISA and acceptable limits of agreement. Three different dilutions of a well characterized set of admission samples from uncomplicated and severe malaria patients studied in a low transmission setting gave an area under the receiver operator characteristic curve of 0.844 in terms of identifying severe malaria. Conclusions: These studies show that plasma PfHRP2 can be assessed via a single RDT, with application of a plasma dilution of 1:5 or 1:10 providing useful diagnostic information to assist in patient management or clinical trial inclusion.en_US
dc.identifier.citationMalaria Journal. Vol.14, No.1 (2015)en_US
dc.identifier.doi10.1186/s12936-015-0900-3en_US
dc.identifier.issn14752875en_US
dc.identifier.other2-s2.0-84942085894en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/36073
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84942085894&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleUse of a rapid test to assess plasma Plasmodium falciparum HRP2 and guide management of severe febrile illnessen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84942085894&origin=inwarden_US

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