Publication: A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species
Accepted Date
2012-10-26
Issued Date
2013
Resource Type
Language
eng
ISSN
1935-2735 (electronic)
1935-2727 (printed)
1935-2727 (printed)
Rights
Mahidol University
Bibliographic Citation
Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Bailey MS, Holden MT, et al. A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. PLoS Negl Trop Dis. 2013;7(1):e1954.
Suggested Citation
Siriphan Boonsilp, ศิริพรรณ บุญศิลป์, Janjira Thaipadungpanit, จันทรจิรา ไทยผดุงพานิช, Premjit Amornchai, เปรมจิตร อมรชัย, Vanaporn Wuthiekanun, วรรณพร วุฒิเอกอนันต์, Bailey, Mark S., Holden, Matthew T. G., Zhang, Cuicai, Jiang, Xiugao, Koizumi, Nobuo, Taylor, Kyle, Galloway, Renee, Hoffmaster, Alex R., Craig, Scott, Smythe, Lee D., Hartskeerl, Rudy A., Day, Nicholas P., Narisara Chantratita, นริศรา จันทราทิตย์, Feil, Edward J., Aanensen, David M., Spratt, Brian G., Peacock, Sharon J. A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Bailey MS, Holden MT, et al. A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. PLoS Negl Trop Dis. 2013;7(1):e1954.. doi:10.1371/journal.pntd.0001954 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/682
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Title
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species
Author(s)
Siriphan Boonsilp
ศิริพรรณ บุญศิลป์
Janjira Thaipadungpanit
จันทรจิรา ไทยผดุงพานิช
Premjit Amornchai
เปรมจิตร อมรชัย
Vanaporn Wuthiekanun
วรรณพร วุฒิเอกอนันต์
Bailey, Mark S.
Holden, Matthew T. G.
Zhang, Cuicai
Jiang, Xiugao
Koizumi, Nobuo
Taylor, Kyle
Galloway, Renee
Hoffmaster, Alex R.
Craig, Scott
Smythe, Lee D.
Hartskeerl, Rudy A.
Day, Nicholas P.
Narisara Chantratita
นริศรา จันทราทิตย์
Feil, Edward J.
Aanensen, David M.
Spratt, Brian G.
Peacock, Sharon J.
ศิริพรรณ บุญศิลป์
Janjira Thaipadungpanit
จันทรจิรา ไทยผดุงพานิช
Premjit Amornchai
เปรมจิตร อมรชัย
Vanaporn Wuthiekanun
วรรณพร วุฒิเอกอนันต์
Bailey, Mark S.
Holden, Matthew T. G.
Zhang, Cuicai
Jiang, Xiugao
Koizumi, Nobuo
Taylor, Kyle
Galloway, Renee
Hoffmaster, Alex R.
Craig, Scott
Smythe, Lee D.
Hartskeerl, Rudy A.
Day, Nicholas P.
Narisara Chantratita
นริศรา จันทราทิตย์
Feil, Edward J.
Aanensen, David M.
Spratt, Brian G.
Peacock, Sharon J.
Corresponding Author(s)
Abstract
BACKGROUND: The available Leptospira multilocus sequence typing (MLST) scheme
supported by a MLST website is limited to L. interrogans and L. kirschneri. Our
aim was to broaden the utility of this scheme to incorporate a total of seven
pathogenic species.
METHODOLOGY AND FINDINGS: We modified the existing scheme by replacing one of the
seven MLST loci (fadD was changed to caiB), as the former gene did not appear to
be present in some pathogenic species. Comparison of the original and modified
schemes using data for L. interrogans and L. kirschneri demonstrated that the
discriminatory power of the two schemes was not significantly different. The
modified scheme was used to further characterize 325 isolates (L. alexanderi [n =
5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29],
L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]).
Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated
that each species corresponded to a discrete clade, and that no strains were
misclassified at the species level. Comparison between genotype and serovar was
possible for 254 isolates. Of the 31 sequence types (STs) represented by at least
two isolates, 18 STs included isolates assigned to two or three different
serovars. Conversely, 14 serovars were identified that contained between 2 to 10
different STs. New observations were made on the global phylogeography of
Leptospira spp., and the utility of MLST in making associations between human
disease and specific maintenance hosts was demonstrated.
CONCLUSION: The new MLST scheme, supported by an updated MLST website, allows the
characterization and species assignment of isolates of the seven major pathogenic
species associated with leptospirosis.