Publication:
Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors

Issued Date

2018-10-01

Resource Type

Article

ISSN

18767753
18735061

DOI

10.1016/j.scr.2018.08.014

Other identifier(s)

2-s2.0-85052436739

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Stem Cell Research. Vol.32, (2018), 43-46

Suggested Citation

Ponthip Potirat, Methichit Wattanapanitch, Pakpoom Kheolamai, Surapol Issaragrisil Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors. Stem Cell Research. Vol.32, (2018), 43-46. doi:10.1016/j.scr.2018.08.014 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45041

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Title

Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors

Author(s)

Ponthip Potirat
 Methichit Wattanapanitch
 Pakpoom Kheolamai
 Surapol Issaragrisil

Other Contributor(s)

Faculty of Medicine, Thammasat University
 Faculty of Medicine, Siriraj Hospital, Mahidol University

Abstract

© 2018 The Authors Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource table [Table presented]

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/45041

Collections

Scopus 2018