Publication: Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling
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Issued Date
2020-01-01
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ISSN
16182650
16182642
16182642
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2-s2.0-85096622413
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Mahidol University
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SCOPUS
Bibliographic Citation
Analytical and Bioanalytical Chemistry. (2020)
Suggested Citation
Duy Ba Ngo, Thanyarat Chaibun, Lee Su Yin, Benchaporn Lertanantawong, Werasak Surareungchai Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling. Analytical and Bioanalytical Chemistry. (2020). doi:10.1007/s00216-020-03061-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/60409
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Title
Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling
Abstract
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature. The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 (HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry (SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 ± 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported. Graphical abstract[Figure not available: see fulltext.].