Publication: Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area
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Issued Date
2003-12-01
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ISSN
00762997
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2-s2.0-5344256895
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Mahidol University
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SCOPUS
Bibliographic Citation
Malacologia. Vol.45, No.1 (2003), 149-166
Suggested Citation
Christopher Rowe, Wannaporn Ittiprasert, Carolyn Patterson, Claudia Eliff, Kristen Page, Susan Bandoni, Thomas Wilke, Dennis Minchella, Fred Lewis, Matty Knight Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area. Malacologia. Vol.45, No.1 (2003), 149-166. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20609
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Title
Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area
Abstract
Genetic variation was assessed in Biomphalaria glabrata snails using variations in microsatellite loci and by RAPD-PCR analysis. Populations of snails examined were field-collected isolates from a small pond in a schistosomiasis endemic region in Brazil, after standard conditions were developed for analyzing snails from two laboratory-maintained stocks. The analyses were performed using a total of 60 microsatellite primer sets and, for RAPD-PCR, a total of 19 random primers. We show that genetic diversity can readily be detected by both molecular methods among the field-collected snails from this small site. In addition, RAPD-PCR bands that were found in another study to segregate with parasite resistance were not detected in any of the field-collected snails analyzed.