Publication: Orthogonal Regulatory Circuits for Escherichia coli Based on the γ-Butyrolactone System of Streptomyces coelicolor
Issued Date
2018-04-20
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21615063
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2-s2.0-85045856227
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Mahidol University
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SCOPUS
Bibliographic Citation
ACS Synthetic Biology. Vol.7, No.4 (2018), 1043-1055
Suggested Citation
Marc Biarnes-Carrera, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, Eriko Takano Orthogonal Regulatory Circuits for Escherichia coli Based on the γ-Butyrolactone System of Streptomyces coelicolor. ACS Synthetic Biology. Vol.7, No.4 (2018), 1043-1055. doi:10.1021/acssynbio.7b00425 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45185
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Title
Orthogonal Regulatory Circuits for Escherichia coli Based on the γ-Butyrolactone System of Streptomyces coelicolor
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Abstract
© 2018 American Chemical Society. Chemically inducible transcription factors are widely used to control gene expression of synthetic devices. The bacterial quorum sensing system is a popular tool to achieve such control. However, different quorum sensing systems have been found to cross-talk, both between themselves and with the hosts of these devices, and they are leaky by nature. Here we evaluate the potential use of the γ-butyrolactone system from Streptomyces coelicolor A3(2) M145 as a complementary regulatory circuit. First, two additional genes responsible for the biosynthesis of γ-butyrolactones were identified in S. coelicolor M145 and then expressed in E. coli BL21 under various experimental conditions. Second, the γ-butyrolactone receptor ScbR was optimized for expression in E. coli BL21. Finally, signal and promoter crosstalk between the γ-butyrolactone system from S. coelicolor and quorum sensing systems from Vibrio fischeri and Pseudomonas aeruginosa was evaluated. The results show that the γ-butyrolactone system does not crosstalk with the quorum sensing systems and can be used to generate orthogonal synthetic circuits.