Publication:
Immunohistochemical study of acute and chronic Toxqplasmosis in experimentally infected mice

dc.contributor.authorPhuangphet Wareeen_US
dc.contributor.authorDavid J P Fergusonen_US
dc.contributor.authorEmsri Pongponratnen_US
dc.contributor.authorUrai Chaisrien_US
dc.contributor.authorYaowalark Sukthanaen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.contributor.otherNaresuan Universityen_US
dc.date.accessioned2018-08-24T02:09:22Z
dc.date.available2018-08-24T02:09:22Z
dc.date.issued2007-03-01en_US
dc.description.abstractAcute and chronic Toxoplasma infections were evaluated in mice using stage specific antibodies and immunocytochemistry. Mice with acute toxoplasmosis were less active, had erectile body hair and seldom took food or water resulting in weight loss. All mice died within 7 days post-inoculation. The immunohistochemical technique enhanced visualization of parasites allowing their distribution to be accurately followed, Following intraperitoneal infection, tachyzoites were initially identified on the surface of the liver and spleen. There was a rapid increase in the number of tachyzoites associated with Invasion from the surrounding connective tissue into the organs with formation of inflammatory lesions in the liver. The focal inflammatory lesions showed increasing numbers of tachyzoites with the period post-inoculation, Similar increases in tachyzoites were observed for the spleen, In contrast, only a few individual tachyzoites were seen in the brain at the final time point. In chronic infections, the mice were asymptomatic but tissue cysts containing large numbers of bradyzoites were observed in all brains with the average number of 295 tissue cysts per half brain and the average cystic size of 46.02 ± 5.08 μm. By histology and immunostaining, the tissue cysts were readily identifiable along with a mild inflammatory cell infiltration into the meninges and perivascular cuffing, Double immunocytochemical labelling confirmed the exclusive presence of tachyzoites during the acute phase and bradyzoites during the chronic phase.en_US
dc.identifier.citationSoutheast Asian Journal of Tropical Medicine and Public Health. Vol.38, No.2 (2007), 223-231en_US
dc.identifier.issn01251562en_US
dc.identifier.other2-s2.0-34249655761en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/24976
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249655761&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleImmunohistochemical study of acute and chronic Toxqplasmosis in experimentally infected miceen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249655761&origin=inwarden_US

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