Publication: Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer
Issued Date
2020-01-01
Resource Type
ISSN
13652761
01407775
01407775
Other identifier(s)
2-s2.0-85094215766
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Fish Diseases. (2020)
Suggested Citation
Onanong Charoenwai, Saengchan Senapin, Ha Thanh Dong, Molruedee Sonthi Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer. Journal of Fish Diseases. (2020). doi:10.1111/jfd.13290 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/59838
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Title
Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer
Abstract
© 2020 John Wiley & Sons Ltd Non-destructive sampling methods offer practical advantages to detection and monitoring of viral pathogens in economically important farmed fish and broodstock. Here, we investigated whether blood, mucus and fin can be used as non-lethal sample sources for detection of scale drop disease virus (SDDV) in farmed Asian sea bass, Lates calcarifer. Detection of SDDV was performed in parallel from three non-destructive and seven destructive sample types, collected from both clinically sick fish and subclinical fish obtained from an affected farm. The results showed that SDDV was detectable in all 10 sample types with the percentage ranging from 20% to 100%. Blood was the best non-destructive sample source exhibited by the fact that it yielded 100% SDDV-positive tests from both sick (n = 12, 95% CI: 69.9–99.2) and clinically healthy fish (n = 4, 95% CI: 39.6%–97.4%) and is considered a “sterile” sample. This study also revealed concurrent infection of SDDV and two ectoparasites Lernanthropus sp. and Diplectanum sp., in all affected fish (n = 8, 95% CI: 46.7–99.3) during the disease outbreak. These ectoparasites also tested positive for SDDV by PCR, indicating that they were potential sample sources for PCR-based detection of SDDV and possibly other viruses infecting Asian sea bass.