Publication: Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA
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Issued Date
2007-05-01
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ISSN
01663542
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2-s2.0-33947422543
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Mahidol University
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SCOPUS
Bibliographic Citation
Antiviral Research. Vol.74, No.2 (2007), 150-155
Suggested Citation
Witoon Tirasophon, Supansa Yodmuang, Wanlop Chinnirunvong, Nongluk Plongthongkum, Sakol Panyim Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA. Antiviral Research. Vol.74, No.2 (2007), 150-155. doi:10.1016/j.antiviral.2006.11.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24555
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Title
Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA
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Abstract
Yellow head virus (YHV) is an invertebrate nidovirus which causes a severe mortality in cultured Penaeus monodon. The mortality may be prevented by prior treatment of shrimps with YHV-protease dsRNA. Whether the YHV infected shrimp might be cured by the dsRNA remains to be investigated. P. monodon injected with 10-6YHV showed a high virus replication and mortality within 2 days. Injection of 25 μg YHV-protease dsRNA at 3, 6, 12 or 24 h post YHV infection showed a strong inhibition of YHV replication up to 12 h. Unrelated dsRNA-GFP showed no inhibition, indicating that the inhibition was nucleic acid sequence specific through RNAi pathway. Shrimp mortality could be prevented at 3 h post YHV infection by the dsRNA, but not at 24 h. These results demonstrate that YHV-protease dsRNA gives therapeutic effect and pave the way to develop a cure for YHV-infected shrimps. © 2006 Elsevier B.V. All rights reserved.