Publication: Assessment of sperm cryodamage and strategies to improve outcome
Issued Date
2000-11-27
Resource Type
ISSN
03037207
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2-s2.0-0034722227
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Cellular Endocrinology. Vol.169, No.1-2 (2000), 3-10
Suggested Citation
Sergio Oehninger, Namik Kemal Duru, Chartchai Srisombut, Mahmood Morshedi Assessment of sperm cryodamage and strategies to improve outcome. Molecular and Cellular Endocrinology. Vol.169, No.1-2 (2000), 3-10. doi:10.1016/S0303-7207(00)00343-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25845
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Title
Assessment of sperm cryodamage and strategies to improve outcome
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Abstract
Sperm cryopreservation still represents a valuable clinical aid in the management of infertility. Its current principal indications include (1) donor sperm insemination; (2) freezing before cancer therapy to maintain reproductive capacity; (3) patient's convenience; and (4) because of the outstanding success with ICSI, even patients with different degrees of oligo-asthenoteratozoospermia can now be offered the use of frozen/thawed sperm for oocyte micromanipulation. Although sperm cryopreservation/thawing and results of insemination and IVF have been consistently good using donor semen, results of infertile men (with or without various degrees of oligoasthenoteratozoospermia) have yielded remarkably lower rates of survival and pregnancy. Freezing/thawing techniques have not been subjected to major changes in the last years, Furthermore, the exact nature of sperm cryodamage still remains to be elucidated. Various aspects of sperm freezing are revisited here (1) development of new technical approaches for cryopreservation; (2) analysis of the stimulatory effect of putative cryoprotectant additives; (3) the use of intrauterine insemination-ready processed samples; and (4) selection and optimization of end-points for analysis of cryodamage. It is expected that advances in such areas will improve significantly the cryopreservation/thawing outcome particularly as related to semen samples of subfertile men. Copyright © 2000 Elsevier Science Ireland Ltd.