Publication: Large-scale SNP discovery through RNA sequencing and SNP genotyping by targeted enrichment sequencing in cassava (Manihot esculenta Crantz)
Issued Date
2014-12-31
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19326203
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2-s2.0-84920465477
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.9, No.12 (2014)
Suggested Citation
Wirulda Pootakham, Jeremy R. Shearman, Panthita Ruang-Areerate, Chutima Sonthirod, Duangjai Sangsrakru, Nukoon Jomchai, Thippawan Yoocha, Kanokporn Triwitayakorn, Somvong Tragoonrung, Sithichoke Tangphatsornruang Large-scale SNP discovery through RNA sequencing and SNP genotyping by targeted enrichment sequencing in cassava (Manihot esculenta Crantz). PLoS ONE. Vol.9, No.12 (2014). doi:10.1371/journal.pone.0116028 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32953
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Title
Large-scale SNP discovery through RNA sequencing and SNP genotyping by targeted enrichment sequencing in cassava (Manihot esculenta Crantz)
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Abstract
© 2014 Pootakham et al. Cassava (Manihot esculenta Crantz) is one of the most important crop species being the main source of dietary energy in several countries. Marker-assisted selection has become an essential tool in plant breeding. Single nucleotide polymorphism (SNP) discovery via transcriptome sequencing is an attractive strategy for genome complexity reduction in organisms with large genomes. We sequenced the transcriptome of 16 cassava accessions using the Illumina HiSeq platform and identified 675,559 EST-derived SNP markers. A subset of those markers was subsequently genotyped by capture-based targeted enrichment sequencing in 100 F1 progeny segregating for starch viscosity phenotypes. A total of 2,110 non-redundant SNP markers were used to construct a genetic map. This map encompasses 1,785 cM and consists of 19 linkage groups. A major quantitative trait locus (QTL) controlling starch pasting properties was identified and shown to coincide with the QTL previously reported for this trait. With a high-density SNP-based linkage map presented here, we also uncovered a novel QTL associated with starch pasting time on LG 10.