Publication: HMP-S7 is a novel anti-leukemic peptide discovered from human milk
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Issued Date
2021-08-01
Resource Type
ISSN
22279059
Other identifier(s)
2-s2.0-85113725784
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biomedicines. Vol.9, No.8 (2021)
Suggested Citation
Wararat Chiangjong, Jirawan Panachan, Thitinee Vanichapol, Nutkridta Pongsakul, Pongpak Pongphitcha, Teerapong Siriboonpiputtana, Tassanee Lerksuthirat, Pracha Nuntnarumit, Sarayut Supapannachart, Chantragan Srisomsap, Jisnuson Svasti, Suradej Hongeng, Somchai Chutipongtanate HMP-S7 is a novel anti-leukemic peptide discovered from human milk. Biomedicines. Vol.9, No.8 (2021). doi:10.3390/biomedicines9080981 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76077
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Title
HMP-S7 is a novel anti-leukemic peptide discovered from human milk
Abstract
Chemotherapy in childhood leukemia is associated with late morbidity in leukemic survivors, while certain patient subsets are relatively resistant to standard chemotherapy. It is therefore important to identify new agents with sensitivity and selectivity towards leukemic cells, while having less systemic toxicity. Peptide-based therapeutics has gained a great deal of attention during the last few years. Here, we used an integrative workflow combining mass spectrometric peptide library construction, in silico anticancer peptide screening, and in vitro leukemic cell studies to discover a novel anti-leukemic peptide having 3+ charges and an alpha helical structure, namely HMP-S7, from human breast milk. HMP-S7 showed cytotoxic activity against four distinct leukemic cell lines in a dose-dependent manner but had no effect on solid malignancies or representative normal cells. HMP-S7 induced leukemic cell death by penetrating the plasma membrane to enter the cytoplasm and cause the leakage of lactate dehydrogenase, thus acting in a membranolytic manner. Importantly, HMP-S7 exhibited anti-leukemic effects against patient-derived leukemic cells ex vivo. In conclusion, HMP-S7 is a selective anti-leukemic peptide with promise, which requires further validation in preclinical and clinical studies.