Publication: Detection and typing of human papilloma virus DNAs in normal cervix, intraepithelial neoplasia and cervical cancer in Bangkok
Issued Date
1997-12-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0031301007
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.4 (1997), 707-710
Suggested Citation
Sontana Siritantikorn, Somsak Laiwejpithaya, Uamporn Siripanyaphinyo, Prasert Auewarakul, Pa Thai Yenchitsomanus, Kleophant Thakernpol, Chantapong Wasi Detection and typing of human papilloma virus DNAs in normal cervix, intraepithelial neoplasia and cervical cancer in Bangkok. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.4 (1997), 707-710. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18027
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Detection and typing of human papilloma virus DNAs in normal cervix, intraepithelial neoplasia and cervical cancer in Bangkok
Other Contributor(s)
Abstract
We detected and typed HPV-DNA by polymerase chain reaction (PCR) in cervico-vaginal lavages of 102 women with normal cervical cytology, 57 patients with cervical intraepithelial neoplasia (CIN), and 23 cervical cancer patients. HPV-DNA detection and typing by in situ hybridization were also performed in cervical biopsies from CIN lesions and cancers. Five percent of women with normal cervical cytology, 46% of CIN, and 61% of cervical cancer were positive for HPV-DNA. Of CIN cases with positive HPV-DNA, 69, 15, 8, 4 and 4% were HPV-16, -33, -18, -11 and -16/33 respectively. Of cervical cancer cases with positive HPV-DNA, 86% were HPV-16, 7% were HPV-16/33, 7% were HPV-18/31. HPV typing was performed in biopsies from 37 CIN and 18 cervical cancers by In situ hybridization. By this method, 38% of CIN were HPV-DNA positive, of which 71% were HPV-16 and 7% were each of HPV-11, -18, -31 and -33. Thirty-nine percent of cevical cancers were positive, of which 71% and 29% were HPV-16 and HPV-16/18 respectively.