Publication: Molecular identification of Paragonimus species by DNA pyrosequencing technology
Issued Date
2013-06-01
Resource Type
ISSN
18730329
13835769
13835769
Other identifier(s)
2-s2.0-84876327210
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.62, No.3 (2013), 341-345
Suggested Citation
Chairat Tantrawatpan, Pewpan M. Intapan, Penchom Janwan, Oranuch Sanpool, Viraphong Lulitanond, Chutatip Srichantaratsamee, Witthaya Anamnart, Wanchai Maleewong Molecular identification of Paragonimus species by DNA pyrosequencing technology. Parasitology International. Vol.62, No.3 (2013), 341-345. doi:10.1016/j.parint.2012.11.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31918
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Title
Molecular identification of Paragonimus species by DNA pyrosequencing technology
Abstract
DNA pyrosequencing for PCR amplicons is an attractive strategy for the identification of microorganisms because of its short time performance for large number of samples. In this study, the primers targeting the fragment of ITS2 region of nuclear ribosomal RNA gene were newly developed for pyrosequencing-based identification of 6 Paragonimus species, Paragonimus bangkokensis, Paragonimus harinasutai, Paragonimus heterotremus, Paragonimus macrorchis, Paragonimus siamensis and Paragonimus westermani. Pyrosequencing determination of 39 nucleotides of partial ITS2 region could discriminate 6 Paragonimus species, and could also detect intra-species genetic variation of P. macrorchis. This DNA pyrosequencing-based identification can be a valuable tool to improve species-level identification of Paragonimus in the endemic areas. © 2012 Elsevier Ireland Ltd.