Publication: Agrobacterium tumefaciens iron superoxide dismutases have protective roles against singlet oxygen toxicity generated from illuminated Rose Bengal
Issued Date
2008-12-01
Resource Type
ISSN
15746968
03781097
03781097
Other identifier(s)
2-s2.0-54249100988
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.289, No.1 (2008), 97-103
Suggested Citation
Panatda Saenkham, Supa Utamapongchai, Paiboon Vattanaviboon, Skorn Mongkolsuk Agrobacterium tumefaciens iron superoxide dismutases have protective roles against singlet oxygen toxicity generated from illuminated Rose Bengal. FEMS Microbiology Letters. Vol.289, No.1 (2008), 97-103. doi:10.1111/j.1574-6968.2008.01382.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18816
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Agrobacterium tumefaciens iron superoxide dismutases have protective roles against singlet oxygen toxicity generated from illuminated Rose Bengal
Other Contributor(s)
Abstract
Singlet oxygen is a highly reactive form of molecular oxygen that is harmful to biological systems. Here, the role of three iron-containing superoxide dismutase (sodB) genes is clearly shown in protecting Agrobacterium tumefaciens against singlet oxygen toxicity. A sodBI mutant was more sensitive to singlet oxygen than both wild-type bacteria and a double sodBII-sodBIII mutant strain. Moreover, a sodBI-sodBII double mutant had higher sensitivity to singlet oxygen than a single sodBI mutant, although the double mutant was comparable to a sodB null mutant. High-level expression of sodBI and sodBII fully complemented the singlet oxygen hypersensitivity phenotype of the sodB null mutant, while high-level expression of sodBIII encoding a periplasmic SOD only partially restored the phenotype. Taken together, our data suggest that SodBI and SodBII have novel protective roles against singlet oxygen toxicity through unknown mechanisms. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.