Publication: Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
Issued Date
2001-11-17
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-0034767704
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.19, No.2 (2001), 139-144
Suggested Citation
P. Auewarakul, V. Paungcharoen, S. Louisirirotchanakul, C. Wasi Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays. Asian Pacific Journal of Allergy and Immunology. Vol.19, No.2 (2001), 139-144. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26553
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Title
Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
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Abstract
We made reporter HIV-1 DNA constructs carrying green fluorescent protein (GFP) gene and exchangeable env of subtype E. The recombinant constructs were used to produce infectious reporter viruses, which induced infected cells to emit green fluorescent light and rendered them easily detectable at single cell level. Because the env in this construct can be easily exchanged, viruses with different antigenic epitopes can be made. We used these reporter viruses to set up a neutralizing antibody assay based on fluorescence reduction by flow cytometric measurement. The result of this new assay correlated with the standard infectivity reduction assay using primary isolates. Because this new assay is faster and much less costly than the standard assay using a p24 endpoint and can be performed in peripheral blood mononuclear cells (PBMC), it provides a useful tool for analysis of HIV-1 immune responses.