Publication: Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape.
Accepted Date
2014-05-26
Issued Date
2014-06-10
Copyright Date
2014
Resource Type
Language
eng
ISSN
1759-6653 (electronic)
Rights
Mahidol University
Rights Holder(s)
PubMed Central
Bibliographic Citation
Croucher NJ, Chewapreecha C, Hanage WP, Harris SR, McGee L, van der Linden M. et al. Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape. Genome Biol Evol. 2014 Jun 10;6(7):1589-602.
Suggested Citation
Croucher, Nicholas J., Chewapreecha, Claire, Hanage, William P., Harris, Simon R., McGee, Lesley, Linde, Mark van der, Song, Jae-Hoon, Ko, Kwan Soo, Lencastre, Herminia de, Turner, Claudia, Yang, Fan, Sa´ -Lea˜o, Raquel, Beall, Bernard, Klugman, Keith P., Parkhill, Julian, Turner, Paul, Bentley, Stephen D. Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape.. Croucher NJ, Chewapreecha C, Hanage WP, Harris SR, McGee L, van der Linden M. et al. Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape. Genome Biol Evol. 2014 Jun 10;6(7):1589-602.. doi:10.1093/gbe/evu120. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/844
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Title
Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape.
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Abstract
The multidrug-resistant Streptococcus pneumoniae Taiwan(19F)-14, or PMEN14, clone
was first observed with a 19F serotype, which is targeted by the heptavalent
polysaccharide conjugate vaccine (PCV7). However, "vaccine escape" PMEN14
isolates with a 19A serotype became an increasingly important cause of disease
post-PCV7. Whole genome sequencing was used to characterize the recent evolution
of 173 pneumococci of, or related to, PMEN14. This suggested that PMEN14 is a
single lineage that originated in the late 1980s in parallel with the acquisition
of multiple resistances by close relatives. One of the four detected serotype
switches to 19A generated representatives of the sequence type (ST) 320 isolates
that have been highly successful post-PCV7. A second produced an ST236 19A
genotype with reduced resistance to β-lactams owing to alteration of pbp1a and
pbp2x sequences through the same recombination that caused the change in
serotype. A third, which generated a mosaic capsule biosynthesis locus, resulted
in serotype 19A ST271 isolates. The rapid diversification through homologous
recombination seen in the global collection was similarly observed in the absence
of vaccination in a set of isolates from the Maela refugee camp in Thailand, a
collection that also allowed variation to be observed within carriage through
longitudinal sampling. This suggests that some pneumococcal genotypes generate a
pool of standing variation that is sufficiently extensive to result in "soft"
selective sweeps: The emergence of multiple mutants in parallel upon a change in
selection pressure, such as vaccine introduction. The subsequent competition
between these mutants makes this phenomenon difficult to detect without deep
sampling of individual lineages.