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Purification and characterisation of keratinase from a thermotolerant feather-degrading bacterium

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dc.contributor.authorWorapot Suntornsuken_US
dc.contributor.authorJunthip Tongjunen_US
dc.contributor.authorPoranee Onnimen_US
dc.contributor.authorHiroshi Oyamaen_US
dc.contributor.authorKanok Ratanakanokchaien_US
dc.contributor.authorThanit Kusamranen_US
dc.contributor.authorKohei Odaen_US
dc.contributor.otherKing Mongkuts University of Technology Thonburien_US
dc.contributor.otherKyoto Institute of Technologyen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-06-21T08:06:14Z
dc.date.available2018-06-21T08:06:14Z
dc.date.issued2005-10-01en_US
dc.description.abstractIsolation and identification of a thermotolerant feather-degrading bacterial strain from Thai soil as well as purification and properties of its keratinase were investigated. The thermotolerant bacterium was identified as Bacillus licheniformis. The keratinase was purified to homogeneity by three-step chromatography. The purified enzyme exhibited a high specific activity (218 U mg-1) with 86-fold purification and 25% yield. The enzyme was monomeric and had a molecular mass of 35 kDa. The optimum pH and temperature for the enzyme were 8.5 and 60°C, respectively. The enzyme activity was significantly inhibited by PMSF and partly inhibited by EDTA and iodoacetamide, but was stimulated by metal ions. It hydrolysed soluble proteins with a relative activity of 4-100% and insoluble proteins, including keratins, with a relative activity of 3-35%. Therefore, the enzyme could improve the nutritional value of meat- and poultry-processing wastes containing keratins, collagen and gelatin. © Springer 2005.en_US
dc.identifier.citationWorld Journal of Microbiology and Biotechnology. Vol.21, No.6-7 (2005), 1111-1117en_US
dc.identifier.doi10.1007/s11274-005-0078-xen_US
dc.identifier.issn09593993en_US
dc.identifier.other2-s2.0-27944468580en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/16196
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=27944468580&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titlePurification and characterisation of keratinase from a thermotolerant feather-degrading bacteriumen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=27944468580&origin=inwarden_US
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