Publication: Immunological analysis of Opisthorchis and Clonorchis antigens
Issued Date
1990-01-01
Resource Type
ISSN
14752697
0022149X
0022149X
Other identifier(s)
2-s2.0-0025146234
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Helminthology. Vol.64, No.2 (1990), 133-138
Suggested Citation
S. Sirisinha, D. Sahassananda Immunological analysis of Opisthorchis and Clonorchis antigens. Journal of Helminthology. Vol.64, No.2 (1990), 133-138. doi:10.1017/S0022149X00012049 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15903
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Title
Immunological analysis of Opisthorchis and Clonorchis antigens
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Abstract
Immunoreactive components of Opisthorchis viverrini and Clonorchis sinensis were analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from other related parasites, together with the metabolic products, were tested for their reactivities with sera from patients with opisthorchiasis and clonorchiasis. A significant cross-reactivity in the ELISA was noted between Opisthorchis and Clonorchis. Immunoblotting and radioimmunoprecipitation analyses showed that the 89-kD protein which was previously shown to be a predominant metabolic product of O. viverrini reacted with sera from both groups of patients. However, an antigen with a molecular weight of 16 kD, apparently a predominant somatic component, appeared to be specific for O. viverrini. © 1990, Cambridge University Press. All rights reserved.