Publication: Improved docosahexaenoic acid production in Aurantiochytrium by glucose limited pH-auxostat fed-batch cultivation
Issued Date
2015-11-01
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ISSN
18732976
09608524
09608524
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2-s2.0-84939627457
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioresource Technology. Vol.196, (2015), 592-599
Suggested Citation
Panyawut Janthanomsuk, Cornelis Verduyn, Somchai Chauvatcharin Improved docosahexaenoic acid production in Aurantiochytrium by glucose limited pH-auxostat fed-batch cultivation. Bioresource Technology. Vol.196, (2015), 592-599. doi:10.1016/j.biortech.2015.08.023 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35694
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Title
Improved docosahexaenoic acid production in Aurantiochytrium by glucose limited pH-auxostat fed-batch cultivation
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Abstract
© 2015 Elsevier Ltd. Fed-batch, pH auxostat cultivation of the docosahexaenoic acid (DHA)-producing microorganism Aurantiochytrium B072 was performed to obtain high cell density and record high productivity of both total fatty acid (TFA) and DHA. Using glucose feeding by carbon excess (C-excess) and by C-limitation at various feeding rates (70%, 50% or 20% of C-excess), high biomass density was obtained and DHA/TFA content (w/w) was improved from 30% to 37% with a 50% glucose feed rate when compared with C-excess. To understand the biochemistry behind these improvements, lipogenic enzyme assays and in silico metabolic flux calculations were used and revealed that enzyme activity and C-fluxes to TFA were reduced with C-limited feeding but that the carbon flux to the polyketide synthase pathway increased relative to the fatty acid synthase pathway. As a result, a new strategy to improve the DHA to TFA content while maintaining relatively high DHA productivity is proposed.