Publication: Herbal drug ninjin'yoeito accelerates myelopoiesis but not erythropoiesis in vitro
Issued Date
2014-01-01
Resource Type
ISSN
13652443
13569597
13569597
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2-s2.0-84898780902
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Mahidol University
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SCOPUS
Bibliographic Citation
Genes to Cells. Vol.19, No.5 (2014), 432-440
Suggested Citation
Tomoko Inoue, Kasem Kulkeaw, Kanitta Muennu, Yuka Tanaka, Yoichi Nakanishi, Daisuke Sugiyama Herbal drug ninjin'yoeito accelerates myelopoiesis but not erythropoiesis in vitro. Genes to Cells. Vol.19, No.5 (2014), 432-440. doi:10.1111/gtc.12143 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33409
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Title
Herbal drug ninjin'yoeito accelerates myelopoiesis but not erythropoiesis in vitro
Abstract
Some Kampo medicines that are herbal and traditional in Japan have had beneficial effects when given to patients with anemia. However, molecular mechanisms underlying their effects are unclear. To address this question, four Kampo medicines used to treat anemia-ninjin'yoeito (NYT), shimotsuto (SMT), juzentaihoto (JTT), and daibofuto (DBT)-were tested separately using in vitro cultures of mouse bone marrow mononuclear cells. Among them, NYT was most effective in stimulating cell proliferation and up-regulating Myc expression. Flow cytometry analysis indicated that, among hematopoietic components of those cultures, myeloid cells expressing CD45/Mac-1/Gr-1/F4/80 increased in number, but Ter119/CD71 erythroid cells did not. Accordingly, real-time PCR analysis showed up-regulation of the myeloid gene Pu.1, whereas the erythroid genes Gata1 and Klf1 were down-regulated. Overall, these findings provide molecular evidence that NYT accelerates myelopoiesis but not erythropoiesis in vitro. © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.