Publication: Control Cells for Both Electrical Impedance and Light Scattering Automated Hematological Analyzers: Preparation from Normal and Thalassemic Blood Samples
Issued Date
1997-09-01
Resource Type
ISSN
01252208
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2-s2.0-0009058783
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.80, No.SUPPL. 1 (1997)
Suggested Citation
Pasra Arnutti, Ahnond Bunyaratavej, Kovit Patanapanyasat, Ampaiwan Chuansumrit Control Cells for Both Electrical Impedance and Light Scattering Automated Hematological Analyzers: Preparation from Normal and Thalassemic Blood Samples. Journal of the Medical Association of Thailand. Vol.80, No.SUPPL. 1 (1997). Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18100
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Title
Control Cells for Both Electrical Impedance and Light Scattering Automated Hematological Analyzers: Preparation from Normal and Thalassemic Blood Samples
Other Contributor(s)
Abstract
The study on quality control of automated blood cell analyzers, Technicon H *1 and Coulter MAXM by using three separately self-prepared control cells was extensively investigated. The three parts of control cells are pseudo-leukocyte and fixed platelets, which are fixed by glutaraldehyde, and control red cells from normal and thalassemic patients preserved and anticoagulated in CPD or CPDA-I. The Technicon H *1 system was based on the principle of light scattering but the Coulter MAXM was based on the principle of electrical impedance for cell counting and measurement. The self-prepared control cells can be satisfactorily utilized as control for each system with statistically significant difference (p < 0.05) for both systems. The expirated dates for control cells are different in both systems and should be determined for each system specifically. The control red cells prepared from thalassemic patients were quite satisfactorily useful as an abnormal control for both systems during this study.