Publication: The cytotoxicity of self-etching primer bonding agents in vitro
Issued Date
2009-03-01
Resource Type
ISSN
10792104
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2-s2.0-59649104998
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Mahidol University
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SCOPUS
Bibliographic Citation
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. Vol.107, No.3 (2009)
Suggested Citation
La ongthong Vajrabhaya, Suwanna Korsuwannawong, Claudia Bosl, Gottifried Schmalz The cytotoxicity of self-etching primer bonding agents in vitro. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. Vol.107, No.3 (2009). doi:10.1016/j.tripleo.2008.11.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27517
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Title
The cytotoxicity of self-etching primer bonding agents in vitro
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Abstract
Objective: This study evaluated the cytotoxicity of 3 self-etching bonding systems: Optibond Solo Plus SE primer, Xeno III, and i Bond. Study design: The test materials were applied on the dentine discs of dentine barrier models in the same way as in the clinical procedures recommended by each manufacturer. 3-D cell culture of Bovine pulp-derived cells transfected with Simian virus 40 Large T antigen and perfusion condition were conducted in this experiment. Cell viability after exposure to the bonding agents was determined by dimethylthiazolediphenyltetrazolium bromide (MTT) assay. Results: The results revealed that cell survival with the above-mentioned bonding agents was 99.66%, 72.59%, and 10.65%, respectively. i Bond is the most toxic material (P < .05). Xeno III is less toxic than i Bond but more toxic than Optibond Solo Plus SE primer (P < .05). Conclusions: Among the 3 test self-etching bonding systems, Optibond Solo Plus SE primer should be selected in cases where the remaining dentin above pulp tissue is 0.5 mm or less. © 2009 Mosby, Inc. All rights reserved.