Publication: Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp
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Issued Date
2014-01-01
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ISSN
10959947
10504648
10504648
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2-s2.0-84891154542
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Mahidol University
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SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.36, No.1 (2014), 52-60
Suggested Citation
Kanokpan Wongprasert, Tawut Rudtanatip, Jantana Praiboon Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp. Fish and Shellfish Immunology. Vol.36, No.1 (2014), 52-60. doi:10.1016/j.fsi.2013.10.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33099
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Title
Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp
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Abstract
Sulfated galactans (SG) were isolated from the red seaweed Gracilaria fisheri (G.fisheri). Chemical analysis revealed SG contains sulfate (12.7%) and total carbohydrate (42.2%) with an estimated molecular mass of 100kDa. Structure analysis by NMR and FT-IR spectroscopy revealed that SG is a complex structure with a linear backbone of alternating 3-linked β-d-galactopyranose and 4-linked 3,6-anhydrogalactose units with partial 6-O-methylate-β-d-galactopyranose and with sulfation occurring on C4 of d-galactopyranose and C6 of l-galactopyranose units. SG treatment enhanced immune parameters including total haemocytes, phenoloxidase activity, superoxide anions and superoxide dismutase in shrimp Penaeus monodon. Shrimp fed with Artemia salina enriched with SG (100 and 200μgml-1) and inoculated with white spot syndrome virus (WSSV) showed a significantly lower mortality rate and lower viral VP 28 amplification and expression than control. The results suggest that SG from G.fisheri exhibits immune stimulatory and antiviral activities that could protect P.monodon from WSSV infection. © 2013 Elsevier Ltd.